The purification and partial characterization of bone
1994-11-11u2002·u2002Matrix proteins were extracted from bovine cortical bone with EDTA/Tris-HCl under non-dissociative conditions at neutral pH. Four distinct bone resorp…
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1994-11-11u2002·u2002Matrix proteins were extracted from bovine cortical bone with EDTA/Tris-HCl under non-dissociative conditions at neutral pH. Four distinct bone resorp…
Get Price2017-4-1u2002·u2002This study has investigated complexation of fisetin, a natural flavonoid, with three types of cyclodextrins to improve its solubility. Sulfobutylether-β-cyclodextrin (SBE-β-CD) showed the highest complexation efficiency while maintaining the in vitro antioxidant activity of fisetin. Addition of 20%v/v ethanol in water improved the amount of solubilized fisetin in the complex 5.9-fold ...
Get PriceFetal bovine mandible-derived osteoblasts were cultured for the purpose of obtaining a spatiotemporal assessment of bone matrix protein expression during in vitro differentiation.
Get Price2020-11-20u2002·u2002Synthetic bone graft materials are needed due to aging societies. Here, biodegradable and versatile recombinant collagen polypeptide scaffolds are engineered to induce bone regeneration in pre ...
Get Price2021-3-9u2002·u2002Fetal bovine serum (FBS) was from PAA Laboratories (Cat. No A15-151, Cölbe, Germany). 10-nm Au particles conjugated to Protein-A were from CMC, UMC Utrecht (Utrecht, The Netherlands). BSA-c was from Aurion (Wageningen, The Netherlands). Silkworm cocoons from Bombyx mori L. were purchased from Tajima Shoji Co., LTD. (Yokohama, Japan).
Get Price2019-5-2u2002·u2002Cells were maintained in a complete DMEM medium with 10% fetal bovine serum. The nano-HAP suspension was diluted to the concentration range of 0.025–0.2 mg mL −1 by using culture media. Cells were inoculated in 96-well plate at a density of 4000 cells per well, then transferred to 37 °C and 5% CO 2 incubator at 95% humidity for 24 hours.
Get PriceA. Preparation of Demineralized Bone . Bovine metatarsal bone was obtained fresh from the slaughterhouse and transported on dry ice. The bones were cleaned of marrow and non-bone tissues, broken in fragments smaller than 1 cm diameter, and pulverized in a mill at 4° C.
Get PriceEvaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system Qian Wang,1–4 Lingyan Cao,4,5 Yang Liu,6 Ao Zheng,4,5 Jiannan Wu,4,5 Xinquan Jiang,4,5 Ping Ji1–3 1Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, 2Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences ...
Get Price2012-12-1u2002·u2002Arca subcrenata Lischke is a marine traditional Chinese medicine. The study investigated the antitumor effects of P2, a polypeptide fraction from A. subcrenata, and its toxicity in vitro and in vivo.
Get PriceOne of the key differences between mature milk and colostrum is the high concentration of IgG found in colostrum, which reaches up to 50-100 mg/mL in the first days after birth (33, 82,83). Bovine ...
Get Price1994-11-11u2002·u2002Matrix proteins were extracted from bovine cortical bone with EDTA/Tris-HCl under non-dissociative conditions at neutral pH. Four distinct bone resorp…
Get Price2017-4-1u2002·u2002This study has investigated complexation of fisetin, a natural flavonoid, with three types of cyclodextrins to improve its solubility. Sulfobutylether-β-cyclodextrin (SBE-β-CD) showed the highest complexation efficiency while maintaining the in vitro antioxidant activity of fisetin. Addition of 20%v/v ethanol in water improved the amount of solubilized fisetin in the complex 5.9-fold ...
Get PriceFetal bovine mandible-derived osteoblasts were cultured for the purpose of obtaining a spatiotemporal assessment of bone matrix protein expression during in vitro differentiation.
Get Price2020-11-20u2002·u2002Synthetic bone graft materials are needed due to aging societies. Here, biodegradable and versatile recombinant collagen polypeptide scaffolds are engineered to induce bone regeneration in pre ...
Get Price2021-3-9u2002·u2002Fetal bovine serum (FBS) was from PAA Laboratories (Cat. No A15-151, Cölbe, Germany). 10-nm Au particles conjugated to Protein-A were from CMC, UMC Utrecht (Utrecht, The Netherlands). BSA-c was from Aurion (Wageningen, The Netherlands). Silkworm cocoons from Bombyx mori L. were purchased from Tajima Shoji Co., LTD. (Yokohama, Japan).
Get Price2019-5-2u2002·u2002Cells were maintained in a complete DMEM medium with 10% fetal bovine serum. The nano-HAP suspension was diluted to the concentration range of 0.025–0.2 mg mL −1 by using culture media. Cells were inoculated in 96-well plate at a density of 4000 cells per well, then transferred to 37 °C and 5% CO 2 incubator at 95% humidity for 24 hours.
Get PriceA. Preparation of Demineralized Bone . Bovine metatarsal bone was obtained fresh from the slaughterhouse and transported on dry ice. The bones were cleaned of marrow and non-bone tissues, broken in fragments smaller than 1 cm diameter, and pulverized in a mill at 4° C.
Get PriceEvaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system Qian Wang,1–4 Lingyan Cao,4,5 Yang Liu,6 Ao Zheng,4,5 Jiannan Wu,4,5 Xinquan Jiang,4,5 Ping Ji1–3 1Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, 2Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences ...
Get Price2012-12-1u2002·u2002Arca subcrenata Lischke is a marine traditional Chinese medicine. The study investigated the antitumor effects of P2, a polypeptide fraction from A. subcrenata, and its toxicity in vitro and in vivo.
Get PriceOne of the key differences between mature milk and colostrum is the high concentration of IgG found in colostrum, which reaches up to 50-100 mg/mL in the first days after birth (33, 82,83). Bovine ...
Get PriceJapan). In brief, cells were seeded in a 96‑well plate at a concentration of 5x103 cells/100 µl/well in complete media without M‑CSF and incubated with different concentrations of purified peptide fractions for 24 or 48 h. A total of 10 µl CCK‑8 reagent was directly added to each well and the incuba-tion was continued for an additional 3 h.
Get PriceLarge-scale and fast synthesis of nano-hydroxyapatite powder by a microwave-hydrothermal method† Zhengwei Cai, ab Xinyu Wang, *ab Zongrui Zhang,c Yingchao Han,*ab Jing Luo,ab Mingzheng Huang,ab BoWen Zhangab and Yuanjing Houab A microwave-hydrothermal (M-H) method assisted with ultrasonic atomization precipitation was
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Get Price2012-5-11u2002·u2002Cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (Hyclone Laboratories, Logan, UT). After deprivation of serum, B104 cells were treated with TGF-β1 (R&D Systems, Minneapolis, MN) or SB431542 (Sigma) for the indicated times, and then proteins or total RNA were extracted.
Get Price2019-7-10u2002·u2002Then, the mononuclear cells were suspended in a complete culture medium (CCM) consisting of α-MEM with GlutaMax, 10% fetal bovine serum (10099141), 100 U ml −1 penicillin, and 100 μg ml −1 streptomycin (15140122) (all from Gibco), and cultured at 37 °C with 5% CO 2. The medium was replaced every 72 h and the non-adherent cells were removed.
Get Price2019-7-30u2002·u2002Research Article The Extract of Chrysanthemum zawadskii var. latilobum Ameliorates Collagen-Induced Arthritis in Mice A-RamKim, 1 HyukSoonKim, 1 DoKyunKim, 1 JunHoLee, 1 YoungHyoYoo, 2 JeomYongKim, 2 SunKyuPark, 2 SeungTaekNam, 1 HyunWooKim, 1 YoungHwanPark, 1 DajeongLee, 1 MinBeomLee, 1 YoungMiKim, 3 andWahnSooChoi 1 Department of Immunology, …
Get PriceWelcome To Longdom Publishing SL. Longdom Publishing SL is one of the leading international publishers of open access journals covering clinical, medical, biological, pharmaceutical sciences as well as engineering, management and technology oriented subjects. Our aim is to catalogue the current scholarly information sourced from across the world and broadcast to maximize its utility and impact ...
Get Price2019-11-26u2002·u20021 Introduction. Bone cancer is a general term for malignant bone tumors such as osteosarcoma, chondrosarcoma, and fibrosarcoma. 1 It is usually divided into autologous skeletal system cancer and bone-metastases cancer (primary tumors in breast, lung, and kidney). 2 At present, the treatment of bone cancer usually combines destructive surgery (amputation or comprehensive limb …
Get Price2019-2-11u2002·u2002Weilin Lin et al. report a new assay for measuring the activity of peptidyl prolyl cis/trans isomerase (PPIase) at the cell surface using ultra performance liquid chromatography. They find that ...
Get PriceThe present article describes the state of the art in the rapidly developing field of bone tissue engineering, where many disciplines, such as material science, mechanical engineering, clinical medicine and genetics, are interconnected. The main objective is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair.
Get PriceJapan). In brief, cells were seeded in a 96‑well plate at a concentration of 5x103 cells/100 µl/well in complete media without M‑CSF and incubated with different concentrations of purified peptide fractions for 24 or 48 h. A total of 10 µl CCK‑8 reagent was directly added to each well and the incuba-tion was continued for an additional 3 h.
Get Price2021-3-18u2002·u2002In recent years, strontium-substituted calcium phosphate bone cement (Sr-CPC) has attracted more and more attentions in the field of bone tissue repair due to its comprehensive advantages of both traditional CPC and Sr ions. In this study, a crucial Sr-containing α-Ca3–xSrx(PO4)2 salt has been synthesized using a simplified one-step method at lower synthesis temperature. A novel Sr-CPC has ...
Get PriceProducts list. The product websites presented here are intended for use in the United States, its territories and Puerto Rico only. Other countries may have different regulatory requirements and review practices that may require referencing different information.
Get Price2021-8-28u2002·u2002CLINICAL FEATURES. SSSS is primarily a disease of neonates and children (48, 96, 174), and white children may be more prone to SSSS than black children, according to a study of 75 cases published more than 2 decades ago ().The clinical features of SSSS vary from localized blisters to severe exfoliation affecting over 90% of the entire body surface.
Get PriceIntroduction. Bone is an essential and multifunctional organ. While providing weight-bearing sustainment and assisting locomotion, the 206 bones in the adult human body have other defined biological roles, such as: generation of blood cells (haematopoiesis), physical protection of vital organs like brain or heart, and storage of minerals and growth factors (Clarke, 2008).
Get Price2021-5-28u2002·u2002Diabetic wound (DW) is one of the leading complications of patients having a long history of uncontrolled diabetes. Moreover, it also imposes an economic burden on people suffering from wounds to manage the treatment. The major impending factors in the treatment of DW are infection, prolonged inflammation and decreased oxygen levels. Since these non-healing wounds are associated with an ...
Get Price2020-6-5u2002·u2002Calcium carbonate (29.36 g of powder) was transformed into a calcium nitrate Ca(NO3)2 solution using concentrated nitric acid (HNO3) while stirring vigorously, and this resulted in the discharge of carbon dioxide (CO2) gas. Figure 1. Thermogravimetric analysis of (A) HA sample and (B) mussel shell powder (after being dry at 70 C for a few days).
Get Price2019-7-31u2002·u2002Research Article A Novel Injectable Magnesium/Calcium Sulfate Hemihydrate Composite Cement for Bone Regeneration ShanchuanZhang, 1,2 KeYang, 3 FuzhaiCui, 4 YiJiang, 5 LinglingE, 2 BaohuaXu, 1 andHongchenLiu 2 Center of Stomatology, China-Japan Friendship Hospital, Beijing, China
Get Price2020-9-21u2002·u2002Growth hormone (GH) is a key modulator of growth and GH over-secretion can lead to gigantism. One form is X-linked acrogigantism (X-LAG), in …
Get PriceThe splenocytes were grown in RPMI 1640 medium supplemented with 10 mM HEPES (pH 7.0), non-essential amino acids, 50 M β-mercaptoethanol, 0.03% L-glutamine, 50 g mL −1 gentamycin and 10% heat-inactivated (56°C, 30 min) fetal bovine serum. Bone marrow cell isolation followed the methods described by Randall and Wlodarski et al. .
Get Price2019-5-26u2002·u2002S2 EXPERIMENTAL SECTION Materials. Graphene oxide (GO) was prepared by oxidation and exfoliation of graphite using a modified Hummers method.1 Poly(lactic acid) (PLA) comprising ~2% D-LA with a weight-average molecular weight of 2.23 × 10 5 g/mol and polydispersity index (PDI) of 2.1 was procured from NatureWorks under the trade name of 4032D.
Get Price2018-6-10u2002·u2002The powder mixtures were compressed into dense disks ... fetal bovine serum (FBS), 100Uml−1 penicillin, and 100µgml−1 strep-tomycin. The subcultured cells were seeded at a density of 5600cellscm−2 on all Ca–P ceramics and cultured in the ... (SCMC, Finetec Co Ltd, Tokyo, Japan) gel using hexane, then cooled with dry ice. The frozen
Get Price2021-2-18u2002·u2002The non-labelled cells were centrifuged at 120g for 5 minutes, resuspended in astrocyte medium (DMEM-F12 supplemented with 1% penicillin/streptomycin, 1% glucose, 2% B27, 10% fetal bovine …
Get Price2019-5-31u2002·u2002For the in vivo analysis, different dECMs under the regulation of COL4A2 mixed with PDLSCs and Bio-Oss bone powder were subcutaneously implanted into immunocompromised mice or in defects in rat alveolar bone. The repair effects were identified by histological or …
Get Price2014-10-22u2002·u2002Introduction. Osteosarcoma is a cancer of the bone that primarily affects adolescents. While improvements in treatment have increased the 5-year survival rate to 65%, it still lags behind overall cancer survival rates for that age group. 1 Furthermore, the metastatic or recurring disease 5-year survival rate is still at a meager 20%. 2 Current osteosarcoma therapies include surgical resection ...
Get PriceFor IR analysis, in first 1 mg of the powder samples were carefully mixed with 300 mg of KBr (infrared grade) and palletized under vacuum. Then the pellets were analyzed in the range of 400-4000 cm À1 at the scan speed of 23 scan/min with 4 cm À1 resolution.
Get Price2005-2-1u2002·u2002The type of bone that is commonly found in the posterior maxilla is type III and type IV, according to Lekholm and Zarb.32 Implant failure rate as high as 35% has been reported in type IV bone.33 The amount of bone volume that has been found in type III and IV bone is between 27% and 42%.24,34,35 The percentages found in these studies are similar to the amount of new bone …
Get Price2014-1-28u2002·u2002The precipitated powders were sintered at 400, 650, 800, 1,000, and 1,200 °C to study their thermal stabilities. The sintering process was carried out from room temperature to the pre-determined temperature at a heating rate of 10 °C/min and a dwelling time of 2 h, after which the powder samples were allowed to cool under ambient conditions and preserved in sealed plastic bags for ...
Get Price2020-8-25u2002·u2002Crosslinking Methods. The HT and GA vapor were selected as physical and chemical crosslinking methods for stabilizing the electrospun CET's protein–CS–PEO nanofibers in water. For the HT crosslinking method, the electrospun nanofibers were heated in a vacuum oven at 35–40 °C for 1 h.
Get Price2020-9-21u2002·u2002Growth hormone (GH) is a key modulator of growth and GH over-secretion can lead to gigantism. One form is X-linked acrogigantism (X-LAG), in …
Get PriceNanoparticles Carrying Biological Molecules: Recent Advances and Applications. Abstract. In the past few decades, enormous advances have been achieved in the field of particle technology and the trend has been shifted from macro to micro and recently to the nanoscale. Integration of nanotechnology and biotechnology has paved the way to the ...
Get Price2015-11-20u2002·u2002Bone tissue engineering is important in terms of the treatment of bone defects arising from bone damage associated with a range of diseases, trauma, inflammation, tumour surgery and non-union bone repair following fracturing [].In general, ideal bone replacement materials should be biodegradable, featuring non-toxic degradation products, compatible with the host immune system, supportive with ...
Get Price2012-10-17u2002·u2002Primary bovine chondrocytes were isolated from metacarpophalangeal joints of steers purchased from a meat center in Fukuoka city, Japan using a sequential enzyme digestion method [26]. Full-thickness articular cartilage tissue was harvested from the proximal articular surface of the metatarsal bone and finely diced with a scalpel. The finely ...
Get Price2020-11-18u2002·u2002Dry particles were subjected to scanning electron microscopic (SEM; JSM-IT100 JEOL, Tokyo, Japan), attenuated total reflection Fourier transform-infrared (ATR FT-IR) spectrophotometric (Nicolet 6700, Thermo Electron Corporation, Madison, WI, USA), powder X-ray diffraction (XRD; scanning rate 15°–60° in the 2θ range, scan speed 5° min −1 ...
Get Price2011-11-15u2002·u2002Fetal calf serum (FCS) is a complex nutritional supplement that is routinely used in cell culture media [1, 2].Along with the growth factors, FCS contains several complement proteins [3–5].Proteins of the complement system play a central role in innate immunity [] and when present in cell culture media, they can influence immunological assays [7, 8].
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Get Price2020-5-25u2002·u2002O-028 Customized process models for cell culture processes Harini Narayanan 1, Michael Sokolov 1,2, Alessandro Butte 1,2, Massimo Morbidelli 1,2 1 Institute of Chemical and Bioengineering, ETH Zurich, Switzerland; 2 DataHow AG, Zurich, Switzerland Correspondence: Harini Narayanan ([email protected]) Background. The need for rapid and efficient process development to meet …
Get Price2020-5-30u2002·u2002Environmental consciousness and constraints in developed societies over the past 20 years have brought about a dramatic impact on tannery operations worldwide. Leather industry has been categorized as one of the most polluting industries, and it spoils the continuity of environmental rhythm because of the generation of liquid, solid and gaseous wastes and also by-products.
Get PriceField tests confirm: 10% higher algae output in PBRs with only 1.8mm thin glass tubing. A 10% higher algae output in a glass PBR due to thinner walled... More →. Phenometrics PBR102-F …
Get PriceS(AD)SAD, Size (Area and Depth), Sepsis, Arteriopathy, and Denervation; UT, University of Texas; The morbidity and mortality associated with diabetic foot lesions remain extremely high (), and management needs to be optimized to ensure the best outcome.Although it is accepted that the institution of rapid access to expert multidisciplinary service is an essential component of care (2,3), there ...
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