Fluorescence Assays
Figure 3. Nucleic acid quantitation using Quant-iT Reagents. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/mL calf thymus DNA.(Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation from 20 ng/mL to 1 μg/mL RNA using a 400-fold ...
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