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  • Freezing of cells - replacement of serum by ...

    1991-7-26u2002·u2002Journal of Immunological Methods, 141 (1991) 105-109 105 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 ADONIS 002217599100234E JIM 05999 Freezing of cells - Replacement of serum by oxypolygelatine J. Groth 2, j.

    Get Price
  • Fetal Bovine Serum (FBS)

    Fetal Bovine Serum (FBS) Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications. Explore FBS products Request a sample.

    Get Price
  • Determination of phosphonoformate (foscarnet) in calf

    1999-3-5u2002·u2002Foscarnet was diluted with blank calf serum to make spiked samples of 960 μM and 800 μM, and with blank human serum to make a 800 μM sample. Calibration standards of 3.75, 7.5, 15, 30, 60, 120, 240 and 480 μM were prepared by successive dilution of the 960 μM spiked calf serum with the

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Calf Nutrition from Birth to Breeding - ScienceDirect

    2008-3-1u2002·u2002The ME requirements for maintenance under thermoneutral conditions are approximately 1.75 Mcal/d for a 45-kg calf. Whole milk contains about 5.37 Mcal ME/kg of solids, which means that a 45-kg calf requires about 325 g of milk solids, or 2.6 kg of whole milk (about 2.5 L), just for maintenance.

    Get Price
  • Replacement of serum by insulin and transferrin supports ...

    1980-4-1u2002·u2002The source of CSA was serum-free conditioned medium of GCT cell cultures [9] and was a gift from Dr James Brennan. Results Growth of HL-60 cells in defined medium. In serum-free defined medium, long-term growth of HL-60 cells continues at a rate approx. 80% of that occurring in medium supplemented with serum (fig. 1).

    Get Price
  • Evaluation of the Ruminant Serum Chemistry Profile ...

    2007-11-1u2002·u2002The serum chemistry profile, composed of a battery of tests, is readily available to clinicians through in-house testing or veterinary reference and diagnostic laboratories. A chemistry profile allows for evaluation of several body systems and assessment of metabolic, acid-base, or electrolyte disturbances. The first sections of this article ...

    Get Price
  • Blood pH - an overview

    Eugene Nattie, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. Ventilation and CO 2. Arterial blood pH is the clinically relevant extracellular variable because changes in breathing alter the PaCO 2 directly. The PaCO 2 is determined by the ratio of CO 2 production and alveolar ventilation. For a constant metabolic rate (and CO 2 production), an increase in alveolar ventilation will ...

    Get Price
  • I Flashcards

    A. 'I should provide direct pressure over the site following the injection.' B. 'I should check my blood sugar immediately prior to the administration.' C. 'I should use the abdominal area only for insulin injections.' D. 'I should only use a calibrated insulin syringe for the injections.'

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  • Fetal Bovine Serum (FBS): Past-Present-Future. - Free ...

    2018-1-1u2002·u2002Although bovine sera of alternative origin are available, like newborn calf serum, calf serum, and donor bovine serum, fetal bovine (fetal calf) serum (FBS/FCS) is most widely used due to its low content of immunoglobulins and complement factors (Gstraunthaler and Lindl, 2013).

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  • NL2012.038 Resolving ABO & Rh Blood Group

    2020-2-20u2002·u20022.6.4 NL09-005 Direct Antiglobulin Test 2.6.5 NL2012-033 Preparation of Red Cell Suspensions 3.0 Specimens 3.1 Blood sample collected in EDTA anticoagulant. 3.2 Red cells from clotted samples (SST tubes with gel separator should not be used). 3.3 Venous or capillary blood …

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  • Clinical Transfusion Practice - WHO

    selection guidelines for blood donors has two purposes: firstly, to protect donors from potential harm which may occur as a direct result of the donation process; secondly, to protect recipients of blood transfusion from adverse effects, such as transmission of infectious diseases or other medical

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  • Fetal Bovine Serum

    The country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum …

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  • Serum-free isolation and culture system to enhance the ...

    2015-1-15u2002·u2002Cell therapy using human mesenchymal stem cells (MSCs) is an attractive approach for many refractory diseases. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are considered as a favorable tool due to its abundance in the body, easy proliferation, and high cytokine production potency. In order to avoid the risks associated with the use of fetal bovine serum (FBS) in culture that ...

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  • ANZ Direct Online

    ANZ Direct Online Helpdesk. The ANZ Direct Online Helpdesk is open from 7.30am to 6.00pm, Monday to Friday. You can also send Secure Mail from within the …

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  • Cell Culture Media: A Review - labome.com

    2021-8-26u2002·u2002Presence of serum in the media has many drawbacks and can lead to serious misinterpretations in immunological studies [2, 3].A number of serum-free media have been developed [4, 5].These media are generally specifically formulated to support the culture of a single cell type, such as Knockout Serum Replacement and Knockout DMEM from Thermo Fisher Scientific, and mTESR …

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  • Phenotypic effects of heterozygosity for a BRCA2 mutation ...

    2003-10-15u2002·u2002DT40 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 1 m M L-glutamine, 60 mg/ml benzylpenicillin/100 mg/ml streptomycin solution (Sigma), NaH 2 CO 3 3.7 g/dl, 10% newborn calf serum (GIBCO), 4% chicken serum, 5% tryptose phosphate broth, 0.01 M HEPES pH 7 (Sigma), at 37°C, 10% CO 2, in a humidified incubator.

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  • Code of Practice for the Care and Handling of Beef Cattle

    The result of inadequate colostrum intake is a low concentration of circulating immunoglobulin in the blood of the calf, a condition known as 'failure of passive transfer'. Calves with failure of passive transfer are 1.6 times more likely to become sick and 2.7 times more likely to die before weaning than calves with adequate serum ...

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  • Freezing of cells - replacement of serum by ...

    1991-7-26u2002·u2002Journal of Immunological Methods, 141 (1991) 105-109 105 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 ADONIS 002217599100234E JIM 05999 Freezing of cells - Replacement of serum by oxypolygelatine J. Groth 2, j.

    Get Price
  • Fetal Bovine Serum (FBS)

    Fetal Bovine Serum (FBS) Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications. Explore FBS products Request a sample.

    Get Price
  • Colostrum Supplements and Replacer - Penn State Extension

    2016-5-5u2002·u2002Numerous products designed to replace colostrum are now on the market. These products are made from bovine colostrum or serum and contain 100 to 150 g of IgG per dose. These products also provide fat, protein, vitamins, and minerals needed by the newborn calf, although the amount varies between products. For example, fat content ranges from 6 ...

    Get Price
  • Determination of phosphonoformate (foscarnet) in calf

    1999-3-5u2002·u2002Foscarnet was diluted with blank calf serum to make spiked samples of 960 μM and 800 μM, and with blank human serum to make a 800 μM sample. Calibration standards of 3.75, 7.5, 15, 30, 60, 120, 240 and 480 μM were prepared by successive dilution of the 960 μM spiked calf serum with the

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Calf Nutrition from Birth to Breeding - ScienceDirect

    2008-3-1u2002·u2002The ME requirements for maintenance under thermoneutral conditions are approximately 1.75 Mcal/d for a 45-kg calf. Whole milk contains about 5.37 Mcal ME/kg of solids, which means that a 45-kg calf requires about 325 g of milk solids, or 2.6 kg of whole milk (about 2.5 L), just for maintenance.

    Get Price
  • Replacement of serum by insulin and transferrin supports ...

    1980-4-1u2002·u2002The source of CSA was serum-free conditioned medium of GCT cell cultures [9] and was a gift from Dr James Brennan. Results Growth of HL-60 cells in defined medium. In serum-free defined medium, long-term growth of HL-60 cells continues at a rate approx. 80% of that occurring in medium supplemented with serum (fig. 1).

    Get Price
  • Evaluation of the Ruminant Serum Chemistry Profile ...

    2007-11-1u2002·u2002The serum chemistry profile, composed of a battery of tests, is readily available to clinicians through in-house testing or veterinary reference and diagnostic laboratories. A chemistry profile allows for evaluation of several body systems and assessment of metabolic, acid-base, or electrolyte disturbances. The first sections of this article ...

    Get Price
  • Blood pH - an overview

    Eugene Nattie, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. Ventilation and CO 2. Arterial blood pH is the clinically relevant extracellular variable because changes in breathing alter the PaCO 2 directly. The PaCO 2 is determined by the ratio of CO 2 production and alveolar ventilation. For a constant metabolic rate (and CO 2 production), an increase in alveolar ventilation will ...

    Get Price
  • (PDF) The Main Causes of Calf Mortality in Dairy Farms in ...

    Good calf management practices enhance calf survivability and performance and subsequently, quality replacement stock for the future herd [17]. Important calf management is in housing, feeding and ...

    Get Price
  • Freezing of cells - replacement of serum by ...

    1991-7-26u2002·u2002Journal of Immunological Methods, 141 (1991) 105-109 105 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 ADONIS 002217599100234E JIM 05999 Freezing of cells - Replacement of serum by oxypolygelatine J. Groth 2, j.

    Get Price
  • Fetal Bovine Serum (FBS)

    Fetal Bovine Serum (FBS) Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications. Explore FBS products Request a sample.

    Get Price
  • Colostrum Supplements and Replacer - Penn State Extension

    2016-5-5u2002·u2002Numerous products designed to replace colostrum are now on the market. These products are made from bovine colostrum or serum and contain 100 to 150 g of IgG per dose. These products also provide fat, protein, vitamins, and minerals needed by the newborn calf, although the amount varies between products. For example, fat content ranges from 6 ...

    Get Price
  • Determination of phosphonoformate (foscarnet) in calf and ...

    1999-3-5u2002·u2002Foscarnet was diluted with blank calf serum to make spiked samples of 960 μM and 800 μM, and with blank human serum to make a 800 μM sample. Calibration standards of 3.75, 7.5, 15, 30, 60, 120, 240 and 480 μM were prepared by successive dilution of the 960 μM spiked calf serum …

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Calf Nutrition from Birth to Breeding - ScienceDirect

    2008-3-1u2002·u2002The ME requirements for maintenance under thermoneutral conditions are approximately 1.75 Mcal/d for a 45-kg calf. Whole milk contains about 5.37 Mcal ME/kg of solids, which means that a 45-kg calf requires about 325 g of milk solids, or 2.6 kg of whole milk (about 2.5 L), just for maintenance.

    Get Price
  • Replacement of serum by insulin and transferrin supports ...

    1980-4-1u2002·u2002The source of CSA was serum-free conditioned medium of GCT cell cultures [9] and was a gift from Dr James Brennan. Results Growth of HL-60 cells in defined medium. In serum-free defined medium, long-term growth of HL-60 cells continues at a rate approx. 80% of that occurring in medium supplemented with serum (fig. 1).

    Get Price
  • Evaluation of the Ruminant Serum Chemistry Profile ...

    2007-11-1u2002·u2002The serum chemistry profile, composed of a battery of tests, is readily available to clinicians through in-house testing or veterinary reference and diagnostic laboratories. A chemistry profile allows for evaluation of several body systems and assessment of metabolic, acid-base, or electrolyte disturbances. The first sections of this article ...

    Get Price
  • Blood pH - an overview

    Eugene Nattie, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. Ventilation and CO 2. Arterial blood pH is the clinically relevant extracellular variable because changes in breathing alter the PaCO 2 directly. The PaCO 2 is determined by the ratio of CO 2 production and alveolar ventilation. For a constant metabolic rate (and CO 2 production), an increase in alveolar …

    Get Price
  • (PDF) The Main Causes of Calf Mortality in Dairy Farms in ...

    Good calf management practices enhance calf survivability and performance and subsequently, quality replacement stock for the future herd [17]. Important calf …

    Get Price
  • Freezing of cells - replacement of serum by ...

    1991-7-26u2002·u2002Journal of Immunological Methods, 141 (1991) 105-109 105 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 ADONIS 002217599100234E JIM 05999 Freezing of cells - Replacement of serum by oxypolygelatine J. Groth 2, j.

    Get Price
  • Fetal Bovine Serum (FBS)

    Fetal Bovine Serum (FBS) Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications. Explore FBS products Request a sample.

    Get Price
  • Colostrum Supplements and Replacer - Penn State Extension

    2016-5-5u2002·u2002Numerous products designed to replace colostrum are now on the market. These products are made from bovine colostrum or serum and contain 100 to 150 g of IgG per dose. These products also provide fat, protein, vitamins, and minerals needed by the newborn calf, although the amount varies between products. For example, fat content ranges from 6 ...

    Get Price
  • Determination of phosphonoformate (foscarnet) in calf

    1999-3-5u2002·u2002Foscarnet was diluted with blank calf serum to make spiked samples of 960 μM and 800 μM, and with blank human serum to make a 800 μM sample. Calibration standards of 3.75, 7.5, 15, 30, 60, 120, 240 and 480 μM were prepared by successive dilution of the 960 μM spiked calf serum with the

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Calf Nutrition from Birth to Breeding - ScienceDirect

    2008-3-1u2002·u2002The ME requirements for maintenance under thermoneutral conditions are approximately 1.75 Mcal/d for a 45-kg calf. Whole milk contains about 5.37 Mcal ME/kg of solids, which means that a 45-kg calf requires about 325 g of milk solids, or 2.6 kg of whole milk (about 2.5 L), just for maintenance.

    Get Price
  • Replacement of serum by insulin and transferrin supports ...

    1980-4-1u2002·u2002The source of CSA was serum-free conditioned medium of GCT cell cultures [9] and was a gift from Dr James Brennan. Results Growth of HL-60 cells in defined medium. In serum-free defined medium, long-term growth of HL-60 cells continues at a rate approx. 80% of that occurring in medium supplemented with serum (fig. 1).

    Get Price
  • Evaluation of the Ruminant Serum Chemistry Profile ...

    2007-11-1u2002·u2002The serum chemistry profile, composed of a battery of tests, is readily available to clinicians through in-house testing or veterinary reference and diagnostic laboratories. A chemistry profile allows for evaluation of several body systems and assessment of metabolic, acid-base, or electrolyte disturbances. The first sections of this article ...

    Get Price
  • Blood pH - an overview

    Eugene Nattie, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. Ventilation and CO 2. Arterial blood pH is the clinically relevant extracellular variable because changes in breathing alter the PaCO 2 directly. The PaCO 2 is determined by the ratio of CO 2 production and alveolar ventilation. For a constant metabolic rate (and CO 2 production), an increase in alveolar ventilation will ...

    Get Price
  • (PDF) The Main Causes of Calf Mortality in Dairy Farms in ...

    Good calf management practices enhance calf survivability and performance and subsequently, quality replacement stock for the future herd [17]. Important calf management is in housing, feeding and ...

    Get Price
  • Freezing of cells - replacement of serum by ...

    1991-7-26u2002·u2002Journal of Immunological Methods, 141 (1991) 105-109 105 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 ADONIS 002217599100234E JIM 05999 Freezing of cells - Replacement of serum by oxypolygelatine J. Groth 2, j.

    Get Price
  • Fetal Bovine Serum (FBS)

    Fetal Bovine Serum (FBS) Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications. Explore FBS products Request a sample.

    Get Price
  • Colostrum Supplements and Replacer - Penn State Extension

    2016-5-5u2002·u2002Numerous products designed to replace colostrum are now on the market. These products are made from bovine colostrum or serum and contain 100 to 150 g of IgG per dose. These products also provide fat, protein, vitamins, and minerals needed by the newborn calf, although the amount varies between products. For example, fat content ranges from 6 ...

    Get Price
  • Determination of phosphonoformate (foscarnet) in calf

    1999-3-5u2002·u2002Foscarnet was diluted with blank calf serum to make spiked samples of 960 μM and 800 μM, and with blank human serum to make a 800 μM sample. Calibration standards of 3.75, 7.5, 15, 30, 60, 120, 240 and 480 μM were prepared by successive dilution of the 960 μM spiked calf serum with the

    Get Price
  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Figure 7. Calf survival rates by level of serum IgG. Source: USDA National Animal Health Monitoring System, 1993. Nutrient Requirements. The newborn calf must be fed highly digestible feedstuffs containing adequate levels of high-quality protein, energy, vitamins, and minerals. Protein. Protein provides amino acids used to build body tissues.

    Get Price
  • Calf Nutrition from Birth to Breeding - ScienceDirect

    2008-3-1u2002·u2002The ME requirements for maintenance under thermoneutral conditions are approximately 1.75 Mcal/d for a 45-kg calf. Whole milk contains about 5.37 Mcal ME/kg of solids, which means that a 45-kg calf requires about 325 g of milk solids, or 2.6 kg of whole milk (about 2.5 L), just for maintenance.

    Get Price
  • Replacement of serum by insulin and transferrin supports ...

    1980-4-1u2002·u2002The source of CSA was serum-free conditioned medium of GCT cell cultures [9] and was a gift from Dr James Brennan. Results Growth of HL-60 cells in defined medium. In serum-free defined medium, long-term growth of HL-60 cells continues at a rate approx. 80% of that occurring in medium supplemented with serum (fig. 1).

    Get Price
  • Evaluation of the Ruminant Serum Chemistry Profile ...

    2007-11-1u2002·u2002The serum chemistry profile, composed of a battery of tests, is readily available to clinicians through in-house testing or veterinary reference and diagnostic laboratories. A chemistry profile allows for evaluation of several body systems and assessment of metabolic, acid-base, or electrolyte disturbances. The first sections of this article ...

    Get Price
  • Blood pH - an overview

    Eugene Nattie, in Seldin and Giebisch's The Kidney (Fifth Edition), 2013. Ventilation and CO 2. Arterial blood pH is the clinically relevant extracellular variable because changes in breathing alter the PaCO 2 directly. The PaCO 2 is determined by the ratio of CO 2 production and alveolar ventilation. For a constant metabolic rate (and CO 2 production), an increase in alveolar ventilation will ...

    Get Price
  • (PDF) The Main Causes of Calf Mortality in Dairy Farms in ...

    Good calf management practices enhance calf survivability and performance and subsequently, quality replacement stock for the future herd [17]. Important calf management is in housing, feeding and ...

    Get Price