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  • (PDF) Serum in mammalian cell culture: Weighing the ...

    The most commonly used serum in culture medium to proliferate mammalian cell lines for rabies vaccine production is fetal bovine serum; due to its low …

    Get Price
  • Pertanika J. Sci. & Technol. 26 (1): 71 - 84 (2018 ...

    2018-3-12u2002·u2002pancreas, liver, bone marrow and islet cells, from various mammalian species have the potential to reverse symptoms of diabetes (Wedemeyer et al., 2016). Insulin and Cell Therapies for Diabetes In the early 1920s, the extract of bovine pancreas cells injected in a diabetic patient reduced diabetic signs such as glycosuria and hyperglycemia.

    Get Price
  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of …

    Get Price
  • Acromegaly pathogenesis and treatment

    2009-11-2u2002·u2002IGF1, the polypeptide target hormone for GH, is synthesized in the liver and extrahepatic tissues (principally bone, muscle, and kidney) and also in the pituitary gland itself. IGF1 mediates most of the growth-promoting actions of GH .

    Get Price
  • (PDF) Large-Scale Expansion of Human Mesenchymal Stem Cells

    the use of fetal bovine serum (FBS) at 10 or 15% (v v) con- centration for large-scale expansion of MSCs of which three studies further supplemented the culture medium with basic

    Get Price
  • (PDF) Streptomyces as host for recombinant production of ...

    For that reason, Malaysia is taken as a case study. The NRW problem is an issue for the water utilities in Malaysia as some states lose nearly 60% of their treated water (MWIG 2015).

    Get Price
  • Development and characterization of cell lines from heart ...

    The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 …

    Get Price
  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Transfection was performed by lipofection using 4.5 µg LipofectAMINE per 0.7 µg DNA. After 72 h post transfection, recombinant cells were selected in medium containing 5% (v/v) fetal bovine serum and 400 µg·mL −1 G-418 Sulfate. Resistant colonies were picked randomly and cultured in 24-well plates, seeding one colony per well in 1 mL ...

    Get Price
  • (PDF) A Comparative Study of Metallothionein Gene ...

    Silver (Ag) is a non-vital metal used in a wide variety of applications. Since the time of its wide spread uses, the systems participation in the uptake of silver in the human cells was poorly known.

    Get Price
  • (PDF) Large-Scale Expansion of Human Mesenchymal

    the use of fetal bovine serum (FBS) at 10 or 15% (v v) con- centration for large-scale expansion of MSCs of which three studies further supplemented the culture medium with basic

    Get Price
  • Expression, purification and renaturation of truncated ...

    2015-3-1u2002·u2002Expression of the recombinant protein. Positive colony was selected and used to inoculate 1 L of LB medium containing 50 μg/mL kanamycin.Cells were grown in a shaker at 37 °C until OD 600 reached 0.9. Following induction with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) being added into the culture medium for 3 h at 37 °C with 200 rpm shaking, cells were harvested by centrifugation at ...

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  • Protides of the Biological Fluids, Volume 32 - 1st Edition

    1985-1-1u2002·u2002Purchase Protides of the Biological Fluids, Volume 32 - 1st Edition. Print Book & E-Book. ISBN 9780080317397, 9781483279664

    Get Price
  • International Society of Pharmacovigilance

    2013-1-9u2002·u2002For this reason, most of the countries established their national pharmacovigilance systems to monitor the adverse drug reaction following the disaster of thalidomide in the 1960s. Within this context, the most common procedure to collect and analyze data about ADRs and identify signals is the spontaneous reporting system (SRS). SRS depends on ...

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  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of the antioxidant …

    Get Price
  • Extrinsic Factors Involved in the Differentiation of Stem ...

    2011-6-16u2002·u2002Human liver-derived fetal cells (FH-B-TPN) Manipulation of culture conditions in various experimental settings: Cells cultured with activin A and betacellulin serum-free medium showed upregulation of NeuroD, Nkx22, glucokinase, prohormone convertase 1/3 and downregulation of Pax6, pancreatic polypeptide, glucagon, and liver markers.

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  • Mammalian Transcription Factor ATF6 Is Synthesized as a ...

    2017-10-13u2002·u2002Plasmid Construction. Recombinant DNA techniques were performed according to standard procedures (Sambrook et al., 1989).The ATF6 expression plasmid pCGN-ATF6 (Zhu et al., 1997) was kindly provided by Dr. R. Prywes (Columbia University, New York, NY).This plasmid contains the full-length ATF6 cDNA at the XbaI site of the mammalian expression vector pCGN and thus expresses …

    Get Price
  • Spondias pinnata (L.f.) Kurz Leaf Extract Derived Zinc ...

    2020-8-6u2002·u2002Alexa Fluor®488-Annexin V/dead cell apoptosis kit, fetal bovine serum (FBS), and 0.25% trypsin-EDTA were obtained from Gibco (Thermo Fisher Scientific, USA). Positively charged nylon membrane was purchased from BDH laboratory supplies, England. SYBR Green PCR Master Mix was purchased from TAKARA (Japan).

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  • GIcNAc phosphotransferase of the lysosomal targeting ...

    2003-9-9u2002·u2002Bovine liver phosphodiester α-GlcNAcase was concentrated to 10 μl in a Speed Vac, combined with 30 μl 0.1 M Tris-HCI, pH 7.4, 8 M guanidine-HCI, and 2–4 μl 25 mM DTT and incubated at 50° C. for 1 hour. Iodoacetamide 2.4 μl 50 μM was then added and the incubation was continued for 1 …

    Get Price
  • ...and who has stake in the petri protein business?

    2018-10-16u2002·u2002Most of the preliminary success of lab-grown meat was developed using fetal bovine serum (FBS), a nutrient-rich blood extracted from unborn calves which contains a variety of growth factors ...

    Get Price
  • Frontiers

    Hirudin, an acidic polypeptide secreted by the salivary glands of Hirudo medicinalis (also known as 'Shuizhi' in traditional Chinese medicine), is the strongest natural specific inhibitor of thrombin found so far. Hirudin has been demonstrated to possess potent anti-thrombotic effect in previous studies. Recently, increasing researches have focused on the anti-thrombotic activity of the ...

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  • Illumina

    Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics.

    Get Price
  • Mammalian Transcription Factor ATF6 Is Synthesized as a ...

    2017-10-13u2002·u2002Plasmid Construction. Recombinant DNA techniques were performed according to standard procedures (Sambrook et al., 1989).The ATF6 expression plasmid pCGN-ATF6 (Zhu et al., 1997) was kindly provided by Dr. R. Prywes (Columbia University, New York, NY).This plasmid contains the full-length ATF6 cDNA at the XbaI site of the mammalian expression vector pCGN and thus expresses …

    Get Price
  • Materials

    The present article describes the state of the art in the rapidly developing field of bone tissue engineering, where many disciplines, such as material science, mechanical engineering, clinical medicine and genetics, are interconnected. The main objective is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair.

    Get Price
  • ...and who has stake in the petri protein business?

    2018-10-16u2002·u2002Most of the preliminary success of lab-grown meat was developed using fetal bovine serum (FBS), a nutrient-rich blood extracted from unborn calves which contains a variety of growth factors ...

    Get Price
  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of the antioxidant …

    Get Price
  • Protides of the Biological Fluids, Volume 32 - 1st Edition

    1985-1-1u2002·u2002Purchase Protides of the Biological Fluids, Volume 32 - 1st Edition. Print Book & E-Book. ISBN 9780080317397, 9781483279664

    Get Price
  • Inactivation of Ricin Toxin by Nanosecond Pulsed Electric ...

    2016-1-5u2002·u2002Ricin is one of the most toxic and easily produced plant protein toxin extracted from the castor oil plant and it has been classified as a chemical warfare agent. Here, nanosecond pulsed electric ...

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  • Nibble Innovativeresearch 703-230-8853

    2021-9-19u2002·u2002Perry incomplete pass to people should lose weight. 703-230-8853 Closed robust metal body with occasional bit of swimming with this yarn. Good nights sleep! (703) 230-8853 Supervisor for media literacy is a courtesy to the pub. (703) 230-8853 Front shin protection. Laich was on leave pending the state training site.

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  • GIcNAc phosphotransferase of the lysosomal targeting ...

    2003-9-9u2002·u2002Bovine liver phosphodiester α-GlcNAcase was concentrated to 10 μl in a Speed Vac, combined with 30 μl 0.1 M Tris-HCI, pH 7.4, 8 M guanidine-HCI, and 2–4 μl 25 mM DTT and incubated at 50° C. for 1 hour. Iodoacetamide 2.4 μl 50 μM was then added and the incubation was continued for 1 …

    Get Price
  • (PDF) Serum in mammalian cell culture: Weighing the ...

    The most commonly used serum in culture medium to proliferate mammalian cell lines for rabies vaccine production is fetal bovine serum; due to its low immunoglobulin and other proteins than other ...

    Get Price
  • Pertanika J. Sci. & Technol. 26 (1): 71 - 84 (2018 ...

    2018-3-12u2002·u2002pancreas, liver, bone marrow and islet cells, from various mammalian species have the potential to reverse symptoms of diabetes (Wedemeyer et al., 2016). Insulin and Cell Therapies for Diabetes In the early 1920s, the extract of bovine pancreas cells injected in a diabetic patient reduced diabetic signs such as glycosuria and hyperglycemia.

    Get Price
  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of the antioxidant …

    Get Price
  • Acromegaly pathogenesis and treatment

    2009-11-2u2002·u2002IGF1, the polypeptide target hormone for GH, is synthesized in the liver and extrahepatic tissues (principally bone, muscle, and kidney) and also in the pituitary gland itself. IGF1 mediates most of the growth-promoting actions of GH .

    Get Price
  • (PDF) Large-Scale Expansion of Human Mesenchymal

    the use of fetal bovine serum (FBS) at 10 or 15% (v v) con- centration for large-scale expansion of MSCs of which three studies further supplemented the culture medium with basic

    Get Price
  • (PDF) Streptomyces as host for recombinant production of ...

    For that reason, Malaysia is taken as a case study. The NRW problem is an issue for the water utilities in Malaysia as some states lose nearly 60% of their treated water (MWIG 2015).

    Get Price
  • Development and characterization of cell lines from heart ...

    The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines.

    Get Price
  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Transfection was performed by lipofection using 4.5 µg LipofectAMINE per 0.7 µg DNA. After 72 h post transfection, recombinant cells were selected in medium containing 5% (v/v) fetal bovine serum and 400 µg·mL −1 G-418 Sulfate. Resistant colonies were picked randomly and cultured in 24-well plates, seeding one colony per well in 1 mL ...

    Get Price
  • (PDF) A Comparative Study of Metallothionein Gene ...

    Silver (Ag) is a non-vital metal used in a wide variety of applications. Since the time of its wide spread uses, the systems participation in the uptake of silver in the human cells was poorly known.

    Get Price
  • ...and who has stake in the petri protein business?

    2018-10-16u2002·u2002Most of the preliminary success of lab-grown meat was developed using fetal bovine serum (FBS), a nutrient-rich blood extracted from unborn calves which contains a variety of growth factors ...

    Get Price
  • (PDF) Serum in mammalian cell culture: Weighing the ...

    The most commonly used serum in culture medium to proliferate mammalian cell lines for rabies vaccine production is fetal bovine serum; due to its low immunoglobulin and other proteins than other ...

    Get Price
  • Pertanika J. Sci. & Technol. 26 (1): 71 - 84 (2018 ...

    2018-3-12u2002·u2002pancreas, liver, bone marrow and islet cells, from various mammalian species have the potential to reverse symptoms of diabetes (Wedemeyer et al., 2016). Insulin and Cell Therapies for Diabetes In the early 1920s, the extract of bovine pancreas cells injected in a diabetic patient reduced diabetic signs such as glycosuria and hyperglycemia.

    Get Price
  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of the antioxidant-rich ...

    Get Price
  • Acromegaly pathogenesis and treatment

    2009-11-2u2002·u2002IGF1, the polypeptide target hormone for GH, is synthesized in the liver and extrahepatic tissues (principally bone, muscle, and kidney) and also in the pituitary gland itself. IGF1 mediates most of the growth-promoting actions of GH .

    Get Price
  • (PDF) Large-Scale Expansion of Human Mesenchymal

    the use of fetal bovine serum (FBS) at 10 or 15% (v v) con- centration for large-scale expansion of MSCs of which three studies further supplemented the culture medium with basic

    Get Price
  • (PDF) Streptomyces as host for recombinant production of ...

    For that reason, Malaysia is taken as a case study. The NRW problem is an issue for the water utilities in Malaysia as some states lose nearly 60% of their treated water (MWIG 2015).

    Get Price
  • Development and characterization of cell lines from heart ...

    The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines.

    Get Price
  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Transfection was performed by lipofection using 4.5 µg LipofectAMINE per 0.7 µg DNA. After 72 h post transfection, recombinant cells were selected in medium containing 5% (v/v) fetal bovine serum and 400 µg·mL −1 G-418 Sulfate. Resistant colonies were picked randomly and cultured in 24-well plates, seeding one colony per well in 1 mL ...

    Get Price
  • (PDF) A Comparative Study of Metallothionein Gene ...

    Silver (Ag) is a non-vital metal used in a wide variety of applications. Since the time of its wide spread uses, the systems participation in the uptake of silver in the human cells was poorly known.

    Get Price
  • ...and who has stake in the petri protein business?

    2018-10-16u2002·u2002Most of the preliminary success of lab-grown meat was developed using fetal bovine serum (FBS), a nutrient-rich blood extracted from unborn calves which contains a variety of growth factors ...

    Get Price
  • (PDF) Serum in mammalian cell culture: Weighing the ...

    The most commonly used serum in culture medium to proliferate mammalian cell lines for rabies vaccine production is fetal bovine serum; due to its low …

    Get Price
  • Pertanika J. Sci. & Technol. 26 (1): 71 - 84 (2018 ...

    2018-3-12u2002·u2002pancreas, liver, bone marrow and islet cells, from various mammalian species have the potential to reverse symptoms of diabetes (Wedemeyer et al., 2016). Insulin and Cell Therapies for Diabetes In the early 1920s, the extract of bovine pancreas cells injected in a diabetic patient reduced diabetic signs such as glycosuria and hyperglycemia.

    Get Price
  • Antioxidant-rich leaf extract of Barringtonia racemosa ...

    2021-8-18u2002·u2002Background Barringtonia racemosa is a medicinal plant belonging to the Lecythidaceae family. The water extract of B. racemosa leaf (BLE) has been shown to be rich in polyphenols. Despite the diverse medicinal properties of B. racemosa, information on its major biological effects and the underlying molecular mechanisms are still lacking. Methods In this study, the effect of …

    Get Price
  • Acromegaly pathogenesis and treatment

    2009-11-2u2002·u2002IGF1, the polypeptide target hormone for GH, is synthesized in the liver and extrahepatic tissues (principally bone, muscle, and kidney) and also in the pituitary gland itself. IGF1 mediates most of the growth-promoting actions of GH .

    Get Price
  • (PDF) Large-Scale Expansion of Human Mesenchymal Stem Cells

    the use of fetal bovine serum (FBS) at 10 or 15% (v v) con- centration for large-scale expansion of MSCs of which three studies further supplemented the culture medium with basic

    Get Price
  • (PDF) Streptomyces as host for recombinant production of ...

    For that reason, Malaysia is taken as a case study. The NRW problem is an issue for the water utilities in Malaysia as some states lose nearly 60% of their treated water (MWIG 2015).

    Get Price
  • Development and characterization of cell lines from heart ...

    The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 …

    Get Price
  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Transfection was performed by lipofection using 4.5 µg LipofectAMINE per 0.7 µg DNA. After 72 h post transfection, recombinant cells were selected in medium containing 5% (v/v) fetal bovine serum and 400 µg·mL −1 G-418 Sulfate. Resistant colonies were picked randomly and cultured in 24-well plates, seeding one colony per well in 1 mL ...

    Get Price
  • (PDF) A Comparative Study of Metallothionein Gene ...

    Silver (Ag) is a non-vital metal used in a wide variety of applications. Since the time of its wide spread uses, the systems participation in the uptake of silver in the human cells was poorly known.

    Get Price
  • ...and who has stake in the petri protein business?

    2018-10-16u2002·u2002Most of the preliminary success of lab-grown meat was developed using fetal bovine serum (FBS), a nutrient-rich blood extracted from unborn calves which contains a …

    Get Price