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Viable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of T cells, we have designed a validated multicolor flow cytometry panel, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide an optimized gating strategy for the ...
Get Price2021-4-12u2002·u2002A mixture of 5 × 10 5 spleen cells from a single laboratory rat or 10 5 spleen cells from a single wild brown rat, 3.125 µg/mL peptide, and 20 ng/mL of rat interleukin-2 was cultured in the chamber of Rat interferon-gamma ELISPOT ® according to the manufacturer's instructions …
Get Price2018-12-7u2002·u2002Porcine rotavirus (PoRV) and porcine epidemic diarrhea virus (PEDV) usually co-infect pigs in modern large-scale piggery, which both can cause severe diarrhea in newborn piglets and lead to significant economic losses to the pig industry. The VP7 protein is the main coat protein of PoRV, and the S protein is the main structural protein of PEDV, which are capable of inducing neutralizing ...
Get PriceAnimals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma.Spleen cells were fused with Sp2/0 mouse myeloma cells.The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen.It is specific to amino acids 195-208 on the factor VII/VIIa molecule.
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get PriceCorning Incorporated is one of the world's leading innovators in materials science, with a nearly 170-year track record of life-changing inventions and category-defining products
Get PriceOur R-PE antibody labeling kit enables the direct conjugation of R-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours. R-Phycoerythrin (R-PE) is a …
Get PriceViable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of T cells, we have designed a validated multicolor flow cytometry panel, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide an optimized gating strategy for the ...
Get PriceIn phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea. Bovine viral diarrhea virus (BVDV) is widely distributed in …
Get PriceSeraCare offers best-in-class Quality Control Tools for NGS clinical laboratories and IVD assay development. Develop, validate, and Monitor your assays with confidence. Custom reference materials, controls, and reagents for NGS, molecular and serology, biological materials, and reagents.
Get PriceHepatocellular carcinoma with metastasis to the spleen in a Holstein cow. Jeong WI(1), Do SH, Sohn MH, Yun HS, Kwon OD, Kim TH, Jeong DH, Williams BH, Jeong KS. Author information: (1)College of Veterinary Medicine, Kyungpook National University, Daegu City 702-701, South Korea.
Get PriceThe A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2. In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1). Note: This HT-2 clone A5E cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at ATCC and by the NIST Mouse STR Consortium ...
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get Price2021-9-16u2002·u2002CD45 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 20103-1-AP.
Get PriceWe, Thai Foods Product International Co., Ltd, established in 2000, are a leading manufacturer and exporter of food ingredient, food additive, foods and beverages in powder forms for more than 20 years. Telephone662 543 7178 Address91/38-39 Suwinthawong Rd, Minburi, Bangkok.
Get Price2015-4-9u2002·u2002The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). None of the infected mice exhibited any clinical signs of illness; however, the ...
Get Price2021-7-28u2002·u2002The Application for Export Certificate must be accompanied by the test results, transfer certificate, or product codes described in the Ractopamine Control Program section of these requirements. The Ocean/Air Cargo Container Number(s) and Seal Number(s) must be indicated in the blocks on the FSIS Form 9295-1, and in the 'Remarks' of the ...
Get PriceIntroducing Semantic Reader in Beta. Semantic Reader is an augmented reader with the potential to revolutionize scientific reading by making it more accessible and richly contextual. Try it for select papers. Learn More. G r een AI R o y Schwa. r. tz, Jesse Dodge, N. A. Smith, O. r. en Etzioni 2020 C.
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get PriceCleaved Notch1 (V1744) (D3B8) Rabbit mAb detects endogenous levels of the Notch1 intracellular domain (NICD) only when released by cleavage between Gly1753 and Val1754 (equivalent to Gly1743/Val1744 of murine notch1). The antibody does not recognize full-length Notch1 or Notch1 cleaved at other positions.
Get Price2021-7-30u2002·u2002The OIE on African swine fever and public-private partnerships. Read the report, 'Stop African swine fever (ASF): Public and private partnering for success', published under the umbrella of the Global Framework for the Progressive Control of Transboundary Animal Diseases (GF-TADs) to learn how PPPs are contributing to rapidly and efficiently control the spread of ASF
Get PriceProtection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection : Alisha Wehdnesday Bernardo Reyes, 1 Tran Xuan Ngoc Huy, 1 Son Hai Vu, 1 Hyun Jin Kim, 1 Jin Ju Lee, 2 Jeong Soo Choi, 2 John Hwa Lee, 3 and Suk Kim 1: 1 Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
Get PriceThe Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models. We stand against unfair gain that publishers collect by creating limits to knowledge ...
Get Price2018-4-5u2002·u2002Irradiated iPSCs prevent tumor growth in murine models of breast, lung, and skin cancer. •. iPSC vaccines target shared antigens between iPSCs and cancer cells. •. iPSC vaccines promote a humoral and cell-mediated anti-tumor immune profile. •. As an adjuvant …
Get Price2017-7-14u2002·u2002Gene transfer into normal quiescent human B cells is a challenging procedure. The present study aimed to investigate whether it is possible to increase the levels of transgene expression by using various types of promoters to drive the expression of selected genes‑of‑interest. To produce lentiviral particles, the present study used the 2nd generation psPAX2 packaging vector and the ...
Get Price2016-1-1u2002·u2002Triple-modal imaging magnetic nanocapsules, encapsulating hydrophobic superparamagnetic iron oxide nanoparticles, are formulated and used to magnetically target solid tumours after intravenous administration in tumour-bearing mice. The engineered magnetic polymeric nanocapsules m -NCs are ~200 nm in size with negative Zeta potential and shown ...
Get Price2021-7-28u2002·u2002The Application for Export Certificate must be accompanied by the test results, transfer certificate, or product codes described in the Ractopamine Control Program section of these requirements. The Ocean/Air Cargo Container Number(s) and Seal Number(s) must be indicated in the blocks on the FSIS Form 9295-1, and in the 'Remarks' of the ...
Get PriceThe Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models. We stand against unfair gain that publishers collect by creating limits to knowledge ...
Get Price2017-7-14u2002·u2002Gene transfer into normal quiescent human B cells is a challenging procedure. The present study aimed to investigate whether it is possible to increase the levels of transgene expression by using various types of promoters to drive the expression of selected genes‑of‑interest. To produce lentiviral particles, the present study used the 2nd generation psPAX2 packaging vector and the ...
Get Price2016-1-1u2002·u2002Triple-modal imaging magnetic nanocapsules, encapsulating hydrophobic superparamagnetic iron oxide nanoparticles, are formulated and used to magnetically target solid tumours after intravenous administration in tumour-bearing mice. The engineered magnetic polymeric nanocapsules m -NCs are ~200 nm in size with negative Zeta potential and shown ...
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get PriceHuman and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the ...
Get PriceGaithersburg, MD) according to the manufacturer's instructions. After isolating the RNA, the RNA pellets were frozen and stored at -80˚C until use. Differentially display-polymerase chain reaction (DD-PCR). DD-PCR was performed using the Genefishing™ kit (Seegene Incorp., Seoul, Korea) according to the manufacturer's instruc-tions.
Get PriceThe peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in ...
Get Price2020-1-1u2002·u2002Figure 1 . Validation of LRRC75A-AS1 in cells and tissues by RT-qPCR. (A) FPKM value of LRRC75A-AS1 in MAC-T (M) and MAC-T treated with heat-killed E. coli (M+E) was obtained from RNA sequence data.(B) Expression of LRRC75A-AS1 in eight different tissues was detected by RT-qPCR.(C) LRRC75A-AS1 was detected in nucleus and cytoplasm by RT-qPCR.(D) It was detected that if there …
Get PriceDetailed herein is a simple protocol for the production LV vectors, describing the triple transfection of an LV transfer vector and LV helper plasmids into HEK-293 cells, and the subsequent ...
Get PriceViable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of T cells, we have designed a validated multicolor flow cytometry panel, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide an optimized gating strategy for the ...
Get PriceIn phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea. Bovine viral diarrhea virus (BVDV) is widely distributed in …
Get PriceSeraCare offers best-in-class Quality Control Tools for NGS clinical laboratories and IVD assay development. Develop, validate, and Monitor your assays with confidence. Custom reference materials, controls, and reagents for NGS, molecular and serology, biological materials, and reagents.
Get PriceHepatocellular carcinoma with metastasis to the spleen in a Holstein cow. Jeong WI(1), Do SH, Sohn MH, Yun HS, Kwon OD, Kim TH, Jeong DH, Williams BH, Jeong KS. Author information: (1)College of Veterinary Medicine, Kyungpook National University, Daegu City 702-701, South Korea.
Get PriceThe A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2. In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1). Note: This HT-2 clone A5E cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at ATCC and by the NIST Mouse STR Consortium ...
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get Price2021-9-16u2002·u2002CD45 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 20103-1-AP.
Get PriceWe, Thai Foods Product International Co., Ltd, established in 2000, are a leading manufacturer and exporter of food ingredient, food additive, foods and beverages in powder forms for more than 20 years. Telephone662 543 7178 Address91/38-39 Suwinthawong Rd, Minburi, Bangkok.
Get Price2015-4-9u2002·u2002The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). None of the infected mice exhibited any clinical signs of illness; however, the ...
Get PriceViable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of T cells, we have designed a validated multicolor flow cytometry panel, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide an optimized gating strategy for the ...
Get PriceIn phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea. Bovine viral diarrhea virus (BVDV) is widely distributed in …
Get PriceSeraCare offers best-in-class Quality Control Tools for NGS clinical laboratories and IVD assay development. Develop, validate, and Monitor your assays with confidence. Custom reference materials, controls, and reagents for NGS, molecular and serology, biological materials, and reagents.
Get PriceHepatocellular carcinoma with metastasis to the spleen in a Holstein cow. Jeong WI(1), Do SH, Sohn MH, Yun HS, Kwon OD, Kim TH, Jeong DH, Williams BH, Jeong KS. Author information: (1)College of Veterinary Medicine, Kyungpook National University, Daegu City 702-701, South Korea.
Get PriceThe A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2. In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1). Note: This HT-2 clone A5E cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at ATCC and by the NIST Mouse STR Consortium ...
Get PriceTransfer 1 ml of tissue resuspended in PBS + PIC into the top chamber of a 50 mm medicone (part #340592). Grind tissue for 2 min according to manufacturer's instructions. Collect cell suspension from the bottom chamber of the medicone using a 1 ml syringe and 18 gauge blunt needle. Transfer cell suspension to a 15 ml conical tube and place on ice.
Get Price2021-9-16u2002·u2002CD45 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 20103-1-AP.
Get PriceWe, Thai Foods Product International Co., Ltd, established in 2000, are a leading manufacturer and exporter of food ingredient, food additive, foods and beverages in powder forms for more than 20 years. Telephone662 543 7178 Address91/38-39 Suwinthawong Rd, Minburi, Bangkok.
Get Price2015-4-9u2002·u2002The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). None of the infected mice exhibited any clinical signs of illness; however, the ...
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